23 Jul Non-contact three-dimensional co-culture method for cells
Abstract
The invention relates to the fields like cells and tissue engineering and provides a non-contact three-dimensional co-culture method for cells. According to the method, a calcium alginate gel layer, a chitosan film layer and a calcium alginate gel layer are successively prepared in culture holes of a culture plate, so a sandwich type composite gel is formed; different cells are in a three-dimensional growth state in the calcium alginate gel layer at two sides of a chitosan film with controllable molecular weight cut off in the composite gel, a culture solution is added, so non-contact three-dimensional co-culture of different cells in a same culture system is realized, thereby allowing behaviors like propagation and differentiation of a target cell in the co-culture system to be regulated and controlled. The method provided by the invention has the advantages of simple operation and low cost, can realize three-dimensional co-culture of different cells which may influence each other and furthest simulates a complex in-vivo cell survival environment.
Year: 2013
Country: CN
Doc No: 103103157