23 Jul Nanometer hydroxyapatite/chitosan porous composite scaffold material as well as bionic dialysis mineralization preparation method and application thereof
Abstract
The invention belongs to the technical field of bone tissue engineering, and discloses a nanometer hydroxyapatite/chitosan porous composite scaffold material as well as a bionic dialysis mineralization preparation method and application thereof. The method comprises the following steps that (1) chitosan is dissolved in an acetic acid solution to obtain a chitosan solution; the chitosan solution isput into a container for freezing; freeze drying is performed to obtain an early-period chitosan porous scaffold; (2) the early-period chitosan porous scaffold is soaked in alkali liquid; water washing is performed until the pH is 7 to 8; freeze drying is performed to obtain a chitosan porous scaffold; (3) the chitosan porous scaffold is soaked in an alkaline phosphatase solution; then, the chitosan porous scaffold and the alkaline phosphatase solution are charged into a dialysis bag; the dialysis bag is put into a mixed solution of calcium glycerophosphate and CaCl2; constant-temperature constant-speed stirring is performed for mineralization; taking out, washing and freeze drying are performed to obtain the nanometer hydroxyapatite/chitosan porous composite scaffold material. The nanometer hydroxyapatite/chitosan porous composite scaffold material can be applied to the field of bone tissue engineering, particularly the bone defect repair.
Year: 2018
Country: CN
Doc No: 108478880