23 Jul Myocardial tissue sensor and myocardial tissue chip preparation method
Abstract
The invention discloses a preparation method of myocardial tissue sensor and a myocardial tissue chip, and belongs to the technical fields of tissue engineering, biomedicine engineering and sensor. The myocardial tissue sensor comprises a myocardial tissue chip and auxiliary parts, wherein the auxiliary parts include an upper die, a lower die, an upper electrode, a lower electrode, a sensitive plate, a parallel light source and a data acquisition system. According to the invention, myocardial cells are sequentially printed on a glass sheet coated with N-isopropylacrylamide and polydimethylsiloxane by virtue of a cell printing technology so as to prepare the myocardial tissue chip; the myocardial tissue chip is kept in the auxiliary parts and is subjected to pulsatile cultivation to form myocardial tissues consistent in beating rhythm, and then the myocardial tissues are kept in a culture solution containing a medicine and undergo electrical stimulation; and a contractile change signal is collected to evaluate the effect of the medicine on cardiac muscle, so as to achieve the purposes of drug screening, testing and the like. The myocardial tissue sensor disclosed by the invention has the advantages of simple device, wide scope of testing medicine, easy detection and processing of signal, and the like.
Year: 2015
Country: CN
Doc No: 104931683