23 Jul Mulberry transgenic method based on agrobacterium tumefacien mediated hybernacle injection
Abstract
The invention discloses a mulberry transgenic method based on agrobacterium tumefacien mediated hybernacle injection. According to the method, a trace amount of agrobacterium tumefacien engineering bacterium conversion liquid containing target gene recombinant vectors is directly injected into mulberry hybernacles to sprout; the hybernacles infect hybernacle top end meristematic tissue meristematic cells and hybernacle axillary bud meristematic cells to obtain successfully converted bud meristematic cells; the bud meristematic cells differentiate and grow to obtain transgenic transgenic branches and buds; then, transgenic grafting transgenic seedlings or transgenic mulberry seeds are obtained through the mulberry grafting vegetative propagation or female mulberry fruit and male flower hybridization sexual propagation technology, and further, a great amount of mulberry transgenic seedlings and seeds can be obtained. The method has the advantages that complicated steps such as tissue culture and requirements on sterile operation environment and the like are avoided; the operation is directly carried out on plants, and can be completed in natural environment; the cost is low; the operation is simple; the technical skill can be easily mastered; the repeatability is good; the conversion efficiency is higher; the transgenic plants, seedlings and seeds suitable for the local wild natural conditions can be fast obtained; the method is applicable to various mulberry varieties, and belongs to a novel efficient and practical mulberry transgenic method.
Year: 2015
Country: CN
Doc No: 104878039