23 Jul Mulberry chalcone isomerase gene and applications thereof
Abstract
The invention discloses a sequence of a chalcone isomerase MaCHI gene from a mulberry, a cloning method and applications. The overall length of the gene 2112bp; the gene contains four exons and three introns; the overall length of an opening reading frame (ORF) is 669bp; 219 amino acid is encoded and a 3′-end non-coding region of 290bp is contained. The gene is a key gene in a plant flavonoid synthesized path and is led into the mulberry by adopting a gene engineering method, so that overexpression is realized and the content of the flavonoid in each tissue of the mulberry can be improved. The mulberry chalcone isomerase gene also can be applied in other plants to change the content of the flavonoid in the plant and varieties and lower the cost of extracting the flavonoid in the plant andis beneficial for promoting the development and utilization of the flavonoid in pharmaceutical industries.
Year: 2012
Country: CN
Doc No: 102643846