Methods of manufacturing hydrogel microparticles having living cells, and compositions for manufacturing scaffold for tissue engineering

Methods of manufacturing hydrogel microparticles having living cells, and compositions for manufacturing scaffold for tissue engineering

Abstract

A method of manufacturing hydrogel microparticles comprising one or more species of living cells attached thereon and/or encapsulated therein is provided. The method includes dissolving a hydrogel-forming agent in an aqueous medium to form a solution; suspending one or more species of living cells in the solution to form a cell suspension; dispersing the cell suspension into an organic oil to form a microemulsion; and subjecting the microemulsion to conditions that allow the hydrogel-forming agent to form hydrogel microparticles comprising one or more species of living cells attached thereon and/or encapsulated therein. Composition comprising a mixture of a degradable hydrogel and at least one hydrogel microparticle having one or more species of living cells, and a method of manufacturing a scaffold for tissue engineering are also provided.

Year:  2015

Country:  CN

Doc No:  104582747

 

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