23 Jul Method of utilizing three-dimensional rack seed cell double phase inoculation to construct engineered tissue
Abstract
The present invention belongs to biomedicine engineering and is one method of constructing engineered tissue. The present invention features that biogel liquid is first prepared and them mixed with seed cell to form cell-biogel suspension, which exists first in liquid state and then in solid in certain condition, to realize double phase inoculation of seed cell in 3D rack. The tree parts of seed cell, biogel and 3D rack forms one united integral and this can realize the efficient combination between cell and material without loss or dropping, maintain the 3D homogeneous distribution of cell in material and maintain the initial form of the engineered tissue. The method of the present invention has simple operation, easy control and short culture period.
Year: 2003
Country: CN
Doc No: 1392253