23 Jul Method of testing amentoflavone in preparation of bone tissue engineering seed cells
Abstract
The invention discloses a method of testing amentoflavone (AME) in the preparation of bone tissue engineering seed cells. The method includes: culturing mesenchymal stem cells (MSCs), adding osteogenesis-inducing medium (OIM) or osteogenesis-inducing medium containing different concentrations of amentoflavone, and detecting cell proliferation by an MTT (methyl thiazolyl tetrazolium) method; after the cells completely fuse, adding osteogenesis-inducing medium or osteogenesis-inducing medium containing different concentrations of amentoflavone to carry out culturing, adding a cell lysis solution, detecting activity of alkaline phosphatase with a kit, and measuring protein concentration by a Bio-Rad method; staining the cells with the alkaline phosphatase, and observing and photographing the cells under a microscope; after the cells completely fuse, adding osteogenesis-inducing medium or osteogenesis-inducing medium containing different concentrations of amentoflavone into each hole, and measuring osteocalcin level with an osteocalcin detection kit.
Year: 2015
Country: CN
Doc No: 105176921