23 Jul Method of producing organization engineered cartilage with inner support
Abstract
The present invention relates to medical tissue engineering, and is process of preparing tissue engineering cartilage with inner support for use as permanent prosthesis clinically. The process includes the following steps: making inner support with nondegradable stable medical porous high-density polyethylene MEDPOR, coating the inner support with nonwoven net of degradable polyglycolic acid (PGA) with high biocompatibility to form compound MEDPOR-PGA rack, dropping prepared autologous marrow matrix stem cell suspension to the nonwoven PGA net, in vitro culturing to the inducing solution with DMEM cartilage connecting TGF-beta1,IGF-I and insulin for certain time, and implanting to under skin for 4-6 weeks to constitute the tissue engineering cartilage. The present invention makes it possible to prepare clinically essential permanent implant.
Year: 2008
Country: CN
Doc No: 101134118