23 Jul Method of modifying mesenchymal stem cell by JAM1 gene and application thereof
Abstract
The invention relates to the technical field of stem cell and gene engineering, provides a method of modifying a mesenchymal stem cell by a link adhesion molecule JAM1 gene, and in particular relates to the design of a primer. JAM1cDNA is prepared by taking the total RNA (Ribonucleic Acid) of a human epidermal cell as a template and by amplifying RT-PCR; then, a recombinant human peGFP-N1-JAM1 eukaryotic expression vector is constructed. In order to improve transfection efficiency, a slow virus over-expression plasmid pGC-FU-JAM1-GFP is constructed, and the slow virus is infected with a human embryo mesenchymal stem cell (hMSC). The invention further provides an application of the method in the preparation of the stem cell promoting hair regeneration and promotion of partial skin hair regeneration in cell transplantation. JAM1ov-hMSC is intradermally transplanted to the skin tissue of a three-week nude mouse; under the induction of nude mouse skin microenvironment, the JAM1ov-hMSC is migrated to the hair follicle of the nude mouse, so that the growth period of the hair follicle of the nude mouse is prolonged, the hair follicle structure is improved and hair is regenerated.
Year: 2013
Country: CN
Doc No: 103031278