23 Jul Method of induction and tissue culture for adventitious roots of Euphorbia fischeriana
Abstract
The invention relates to a method of induction and tissue culture for adventitious roots of Euphorbia fischeriana, belonging to the technical field of cell engineering. The method disclosed herein comprises the following steps: (1) seed germination: sterilizing Euphorbia fischeriana seeds with HgCl2, then putting the sterilized seeds on an MS solid medium, and germinating under illumination; (2) induction of callus: putting the young seedlings obtained by the step (1) on an MS solid medium containing 2,4-dichlorphenoxyacetic acid, naphthylacetic acid, 6-benzylamino adenine and coconut juice, and inducing in sterile and dark conditions to form callus; (3) induction of adventitious roots: putting the callus obtained by the step (2) on an MS solid medium containing indolebutyric acid and coconut juice, and inducing in sterile and dark conditions to form adventitious roots; and (4) culture of adventitious roots: putting the adventitious roots obtained by the step (3) on an MS solid medium containing indolebutyric acid to culture. The method has the advantages of simpleness and has high active substance content.
Year: 2013
Country: CN
Doc No: 102870672