23 Jul Method of establishing rat pancreatic duct epithelioid stem cell system
Abstract
The invention relates to a method of establishing a rat pancreatic duct epithelioid stem cell system, and belongs to the field of animal cell (tissue) engineering. An adult rat pancreatic tissue is digested by collagenase, Dextron discontinuous density gradient centrifugation is carried out, serum culture is carried out in RPMI1640, pancreatic duct primary epithelioid stem cells are grown in a manner of being adhered to the wall, clone ring screening is carried out, epithelioid stem cells are purified, trypsin digestive juice is digested and gone down to the future generation to establish the cell system. The rat pancreatic duct epithelioid stem cell system established by adopting the method disclosed by the invention has the characteristics that the cell adherence is polygonal and epithelioid, and the cells grow in a clonal manner and are normal diploid cells; the rat pancreatic duct epithelioid stem cells express proliferating cell nuclear antigen, an octamer-binding transcription factor 4, stem cell key protein, keratin 19 and a neurogenic differentiation factor 2 on the protein level, have multiple differentiation potentials and are free of oncogenicity.
Year: 2014
Country: CN
Doc No: 103881962