23 Jul Method of establishing efficient regeneration system of shoot tips of dendrocalamus hamiltonii
Abstract
The invention relates to a method of establishing an efficient regeneration system of shoot tips of dendrocalamus hamiltonii. The method comprises the following five steps: S1, induced culture of a lateral bud callus; S2, subculture of the callus; S3, differentiation culture of a seedling; S4, rooting culture of the seedling; and S5, hardening-seedling and transplanting of a regenerated plant. The invention relates to a plant tissue culture method which takes the shoot tip of the lateral bud of the dendrocalamus hamiltonii as an explant and is a beneficial attempt of obtaining the inductivity of an efficient embryonic callus obtained through tissue culture by taking the lateral bud of the bamboo as the explant. By adopting the method, the callus can be efficiently induced from the explant to establish an efficient somatic cell regeneration system, thereby creating beneficial conditions for genetic transformation and cell engineering research of the dendrocalamus hamiltonii and laying a good foundation for breeding by mean of the transgenic technology. The method is simple and feasible, beneficial to popularize, loose in implementing condition and remarkable in effect.
Year: 2014
Country: CN
Doc No: 103782909