23 Jul Method of building new high-efficiency biocatalyst based on tissue engineering method
Abstract
The invention relates to a new method of building a new high-efficiency biocatalyst through the combination of tissue engineering and chemical technology. The method comprises the following steps: using microorganisms as seed cells to expand culture, inoculating on scaffold material, performing tissue culture to prepare a tissue engineering cell sheet with the structure of cell-scaffold three-dimensional space complex, wherein the cell capacity is up to over 80% and enough channels are left in the scaffold for substrate or product to pass in and out; coating the cell sheet with polyvinyl alcohol/sodium alginate, forming in calcium chloride solution, freezing and thawing to form a gelatin coated tablet, wherein partial calcium alginate in the coating layer is dissolved in phosphates to form microchannels used for micromolecular transfer; and then performing permeabilization to increase the endoenzyme in the gelatin coated tablet to build the permeabilized tissue engineering cell gelatin coated tablet which is a new high-efficiency biocatalyst and has high cell capacity, strong catalytic effect, low diffusional limitation and good mechanical strength. The new high-efficiency biocatalyst can be used to replace the traditional immobilized cell and prepare important chiral drugs and pharmaceutical and chemical intermediates such as chiral alcohols through biocatalysis.
Year: 2010
Country: CN
Doc No: 101824407