23 Jul METHOD, KIT, AND APPLICATION OF APPLYING REVERSE DIFFERENTIATION TO HUMAN SOMATIC CELLS TO GENERATE AUTOLOGOUS RETINAL STEM CELLS AND AUTOLOGOUS RETINAL CELLS
Abstract
Provided are a culture solution and a method for reversely differentiating somatic cells to generate autologous retinal stem cells, and the application thereof. The culture solution for reversely differentiating somatic cells to generate autologous retinal stem cells consists of a mixture of multiple plant extracts and multiple proteins, and can perform reverse induced differentiation on human somatic cells such as human peripheral blood mononuclear cells to generate within weeks tens of billions of first generation autologous retinal stem cells and autologous retinal cells. The method has the advantages that the number of the produced retinal stem cells is large, and the quality of the stem cells can be well monitored. The plant and protein induced human autologous retinal stem cells have similar cell specific phenotypes and a variety of cell differentiation capability as the natural retinal stem cells, can undergo differentiation to form light-sensitive cells, the retinal pigment epithelial cells, and the retinal ganglion cells, are not only applicable to treatment of various eye diseases but also applicable to establishment of the autologous retinal stem cell bank to preserve autologous retinal stem cells permanently, and as autologous seed cells and cells for regeneration and repair, have desirable potential applications in treatment of eye diseases, autologous retinal tissue and organ engineering, and the industrial field of the production of the autologous retinal stem cells.
Year: 2013
Country: WO
Doc No: 2013071469