23 Jul Method for wheat tissue culture
Abstract
The invention discloses a method for wheat tissue culture, which comprises the following steps: firstly, performing induction culture on a wheat germ to obtain an embryogenic callus; and secondly, performing differentiation culture on the embryogenic callus to obtain a wheat regenerated plantlet, wherein the method of the induction culture comprises the steps of successively performing induction cultures on the wheat germ with a culture medium containing auxin, a culture medium without the auxin, and the culture medium containing the auxin again to obtain the embryogenic callus. The method for the wheat tissue culture further improves the regeneration frequency of immature embryos and mature embryos of wheat, weakens the barriers caused by the conditions such as genotypes, physiological states and the like to the regeneration, provides a technical support for the cell engineering breeding, the genetic engineering breeding and the functional genomics research of the wheat, and has great significance for the biotechnology breeding and the functional genomics research of the wheat.
Year: 2010
Country: CN
Doc No: 101703004