23 Jul Method for using umbilical stalk placenta to prepare mesenchyma stem cell
Abstract
The invention discloses a method for preparing a mesenchymal stem cell by using umbilical cords and placenta, and mainly relates to a new source for the mesenchymal stem cell and a preparation method thereof. The umbilical cord or the placenta is employed by the stem cell as a cell source; tissues are assimilated at first; then a single cell suspension is prepared and inoculated into a culture bottle, a culture dish or a culture bag to amplify the mesenchymal stem cell, then amplified the mesenchymal stem cell is assimilated, collected and frozen. The mesenchymal stem cell prepared according to the technique of the invention contains a plurality of mesenchymal stem cells with excellent multiplication potentiality. The mesenchymal stem cell can be used in the experimental study, clinical treatment and tissue engineering, etc. The material source thereof is abundant; the separation successful rate and yield are high; the cell multiplication capacity is strong; and the contents of allergic and animal-originated substances are low.
Year: 2009
Country: CN
Doc No: 101492654