23 Jul Method for using centrifugal planting to construct tissue engineering bladder
Abstract
The invention provides a method for using centrifugal planting to construct a tissue engineering bladder. The method comprises the steps of using centrifugal planting equipment to prepare a bladder acellular matrix-seed cell suspension liquid structure; placing the bladder acellular matrix-seed cell suspension liquid structure in a centrifugal machine, wherein the centrifugal machine conducts centrifugal running for a specified time at a specified rotation speed; placing a centrifuged bladder acellular matrix in a culture dish, and using the cultured bladder acellular matrix to construct the tissue engineering bladder. The centrifugal planting equipment is constructed, and the proper rotation speed and the centrifugal planting time of the centrifugal machine are sought. Compared with common static planting and dynamic planting modes like pressure perfusion planting and rotary flask planting, centrifugal planting needs fewer cells; meanwhile, through centrifugal force, the seed cells can be uniformly distributed on the inner wall of a support, so that the planting cells enter the bladder acellular matrix, three-dimensional planting is formed, and the prepared tissue engineering bladder more fits actual bladder tissue.
Year: 2018
Country: CN
Doc No: 108379661