23 Jul Method for tissue culture and rapid propagation of camellia oleifera
Abstract
The invention provides a method for tissue culture and rapid propagation of camellia oleifera. The method for tissue culture and rapid propagation of the camellia oleifera comprises the steps of explant sterilization, primary culture, subculture, rooting culture and transplant. A preparation method of a rooting medium comprises the steps that a 1/2 MS agar culture medium containing 0.5mg / L IBA is prepared, wherein agar is 7-10 g / L, and the PH value is 5.0-5.5; pulverized perlite is well wrapped by means of a net bag and then is placed into a molten culture medium, the culture medium is fully absorbed by the perlite, then the perlite is drained off slightly and sterilized, and then the rooting medium is obtained. According to the method for tissue culture and rapid propagation of the camellia oleifera, the perlite with a certain granularity is used as the culture medium, the culture medium and the agar culture medium are mixed to form the rooting medium, therefore, the root system of the camellia oleifera can breathe fully, the rooting percentage of camellia oleifera cultivation is greatly improved, the root system is developed, the survival rate of transplanting is high, and a technical base is provided for industrialized seedling production and genetic engineering improvement of tissue culture of the camellia oleifera.
Year: 2013
Country: CN
Doc No: 103461143