23 Jul Method for separating and culturing cells from synovial articular cartilage of old people
Abstract
The invention relates to a method for separating and culturing cells from synovial articular cartilage of old people, and belongs to the technical field of human tissue engineering. A large number ofcells with metabolic activity are obtained from senile articular cartilage, and the method is applied to molecular pathology and biological treatment fundamental researches for repairing cartilage tissue defects and cartilage degenerative changes through clinical autologous cartilage cell transplantation. The invention discloses a chondrocyte separation method. According to the method, mechanicalcutting and enzymatic hydrolysis of cartilage tissues are combined. Cartilage fragments are in full contact with degradable cartilage extracellular matrix protein specific protease in a tissue decomposition bottle (Spiner flasK) sterilized at high temperature and high pressure and in a cell incubator, thus achieving the purpose of completely dissolving a matrix to release cartilage cells to the maximum extent. Compared with an existing human load-bearing synovial articular cartilage cell separation method, the method has the advantages that the yield of cartilage cells is higher, the proportion of living cartilage cells is larger and the pollution probability is lower.
Year: 2019
Country: CN
Doc No: 110484495