23 Jul Method for screening rice tissue specific expression type cis-acting element and flanking sequence thereof
Abstract
The invention belongs to the technical field of plant genetic engineering and specifically relates to a method for screening a rice tissue specific expression type cis-acting element and a flanking sequence thereof. According to the information in a rice whole-growth-period expression profile chip database, all tissue specific expression genes are classified according to expression patterns. According to the information in a plant cis-acting element database, cis-acting elements in the promoter regions of different rice tissue specific expression genes are scanned and counted. After that, cis-acting elements in the promoter regions of green tissue specific expression genes, which occur at a high frequency, are selected as candidate elements related to green tissue specific expression. Meanwhile, the flanking sequence of one candidate element GEAT is subjected to bioinformatic prediction. The combination of the above element and the flanking sequence thereof is integrated with -46 Minimal 35S promoter, so that the gene expression of a GUS gene is driven for function identification. The GUS analysis shows that, the GUS gene is associated with the green tissue specific expression. Through the site-directed mutagenesis experiment of the flanking sequence, a basic group having a critical role in maintaining the function of GEAT is found.
Year: 2017
Country: CN
Doc No: 106566825