23 Jul Method for repolarizing primary hepatocytes in vitro
Abstract
The invention relates to a method for repolarizing primary hepatocytes in vitro. The method is characterized by comprising the following steps of planting a primary hepatocyte suspension into a cell culture insert in a perfusion planting mode; meanwhile, applying a compact force to the primary hepatocytes for enabling cell density in a unit volume to reach over 90 percent; then performing perfusion culture by using a serum-free medium with a hepatocyte phenotype. According to the method disclosed by the invention, a primary mature hepatocyte population is planted in a compact mechanical planting mode, and the repolarization of the hepatocytes in structural and functional mechanics are both formed early at twelfth hour after planting, so that the repolarizing speed of an in vitro hepatocyte population in a three-dimensional environment is improved, and primary mature hepatocytes with an approximate physiological function phenotype are provided for liver tissue engineering.
Year: 2015
Country: CN
Doc No: 105132359