23 Jul Method for regulating pore structure of water-soluble polymer tissue engineering scaffold by use of polyester template
Abstract
The invention discloses a method for regulating the pore structure of a water-soluble polymer tissue engineering scaffold by use of a polyester template. The method comprises the following steps of: adding polylactic acid microspheres or fiber into a water-soluble polymer solution; performing freeze drying; and then removing the template to obtain a porous tissue engineering scaffold. The experimental result shows that: the method can obtain a tissue engineering scaffold with aperture of 100-500 microns, over 90% of porosity and over 700% of swelling degree; the scaffold has the characteristics of controllable aperture, sequential arrangement of holes, good pore connectivity and coexistent big and small pores; the structure with coexistent big and small pores is favorable for inoculating and adhering cells, transporting nutrient substances and discharging metabolic products; and moreover, the research indicates that: the differentiation behavior of the stem cell in the scaffold can be intervened in by adjusting the pore structure of the scaffold.
Year: 2012
Country: CN
Doc No: 102690435