23 Jul METHOD FOR PRODUCING IMMOBILISED BACTERIA CELLS
Abstract
The invention relates to bio-engineering, including eco-engineering, and can be used for developing and producing bacterial medicine-, agriculture- and environment-oriented preparations, including synthesis of different biologically active substances, production of highly-active bio-catalysts based on microorganism and plant immobilised cells and on cultivated animal tissue cells. The inventive method for producing immobilised bacteria cells consists in cultivating a suspended cell bacterial mass, for example Psedo-monas-type bacteria, on a mineral-protein nutrition medium, which contains nitrogen, phosphorus, potassium, sodium and peptone at a pH =7.0 for 48 hours. The bacterial cells cultivated on said nutrition medium are used for producing the immobilised cells in the form of bioflocs. For this purpose, the inventive method also consists in successively introducing peroxide and calcium chloride into the suspension of the thus obtained cells in a quantity of 10-10 mole/l or 0.034-0-038 mole/l, respectively at the culture density of 1 10 kl ml, wherein the reagents are introduced at a suspension temperature of 30 °C, and in agitating the suspension for 5 minutes after the introduction of each reagent, wherein the last reagent being introduced, the flocculation of the bacteria cell biomass and the sedimentation of the bioflocs are carried out.
Year: 2007
Country: WO
Doc No: 2007037770