23 Jul Method for producing human tissue plasmin activator
Abstract
The invention belongs to the field of biological engineering, and particularly relates to a method for producing human tissue plasminogen activator (tPA) in large scale. The method is to use an expression vector composed of regulatory sequence containing a core promoter and cDNA and a BLG signal peptide to prepare a transgenic mouse; the lymphocyte or the mammary epithelial cell of the transgenic mouse is taken and fused with a tumour cell to obtain a transgenic hybridoma; a tPA transgenic hybridoma is induced by prolactin and is screened to obtain a high-expression cell strain; then the expression cell is transfected and screened again to obtain a high-expression hybridoma strain; the high-expression cell is inoculated into the abdominal cavity of the BALB/C mouse in a lactation period; then ascites is collected to obtain the recombinant human tissue plasminogen activator. The production cost thereof is remarkably reduced compared with a cell culture method; the product is restively safe in use; the production flow is simple; and complex instruments and devices are not needed.
Year: 2009
Country: CN
Doc No: 101492662