23 Jul Method for producing camellia oleifera tissue culture seedlings in Hainan region
Abstract
The invention belongs to the technical field of plant tissue culture and relates to a method for producing camellia oleifera tissue culture seedlings in the Hainan region. Camellia oleifera fruit immature cotyledons at a development stage of 170-190 d after flowering serve as explants and nodule embryonic tissues are formed by dedifferentiation induction, and somatic embryos are obtained through multiplication culture and redifferentiation culture and are transferred to a plant regeneration induction medium to be induced to obtain somatic embryo regeneration plants with robust embryos and main roots. The tissue culture is utilized, the immature cotyledons at a camellia oleifera development stage of 180 d after flowering are selected to serve as the explants to perform camellia oleifera tissue culture rapid propagation, a whole system for obtaining camellia oleifera regeneration plants is obtained through a somatic embryo generating approach, the technical basis is provided for industrialized seedling production of camellia oleifera in the Hainan region, and the material and technical basis is laid for the genetic engineering improvement of camellia oleifera in the Hainan region.
Year: 2016
Country: CN
Doc No: 105230497