23 Jul Method for producing biological active outcome by using tumour cell
Abstract
The invention relates to the establishment of a bioactive protein expression system in the field of biological engineering. The technology can be applied to the preparation of recombinant protein such as certain cell factors, enzyme and antigen protein (vaccine). The technical proposal of the invention comprises the following: a DNA coding sequence of a functional gene and a gene component consisting of an expression vector containing a core promoter/enhancer sequence are applied to prepare a transgenic small (large) mouse, a transgenic tissue cell is obtained from the transgenic small (large) mouse, a cell fusion method is applied to fuse the transgenic tissue cell with a tumor cell to produce a hybridoma cell, and the hybridoma cell is inoculated in an abdominal cavity of the small mouse to produce the recombinant protein in a large quantity. The application of the technology can efficiently produce the recombinant protein on a large scale. The hybridoma cell and an individual mouse are utilized as production media, which have low cost and high yield, and are safe, sanitary and easy to control the quality than other animals. Therefore, the technology has higher application value in the bioindustry.
Year: 2009
Country: CN
Doc No: 101451151