23 Jul METHOD FOR PREPARING TYPE I COLLAGEN AND FOR PREPARING UNIDIRECTIONAL AND MULTIDIRECTIONAL SCAFFOLDS CONTAINING SAME
Abstract
The invention hereby claimed relates to the field of tissue engineering and concerns a method for isolating type I collagen from rat tail and producing multidirectional scaffolds (Pérez et al., 2001). In recent years, the isolating method has been modified and standardised. Likewise, changes have been introduced into the method for obtaining scaffolds from this collagen and a novel method has been developed for producing unidirectional laminar scaffolds. In the optimised method, the animal collagen source is cleaned, cut and suspended in an acetic acid solution until a liquid phase pH between 2.5 and 3.2 is reached, agitating constantly at 4°C. Said suspension is centrifuged and the pH of the supernatant obtained is neutralised with NaOH in order to re-separate the solid and liquid phases. The collagen precipitated in this way is re-precipitated with salts and solubised in acetic acid, and the solution is poured into moulds, frozen at -20°C and freeze-dried. Lastly, the scaffolds obtained are cross-linked and then sterilised with ethylene oxide.
Year: 2016
Country: WO
Doc No: 2016071876