23 Jul Method for preparing tissue engineering epidermis based on collagen
Abstract
The invention discloses a method for preparing tissue engineering epidermis based on collagen. Epidermal stem cells of the human are adopted as seed cells, a 3D cell-free matrix established by cow placenta I-type collagen serves as a support, a CELLnTEC cultivation system with addition of MMPs inhibitor is used, and the active all-layer tissue engineering epidermis is established through a gas-liquid interface separation method. Compared with the prior art, the method has the advantage that due to addition of the MMPs inhibitor marimastat 5nM to a differential medium, shrinkage of the support and the artificial epidermis product is effectively reduced. The I-type cow collagen 3D support is used for being combined with the CELLnTEC cultivation system, proliferation and differentiation of the epidermal stem cells can be maintained, fibroblast is not used, and the workload and the operation steps of the artificial epidermis product in the preparation process are effectively reduced.
Year: 2016
Country: CN
Doc No: 105963795