23 Jul Method for preparing tissue engineering corneal limbus based on Matrigel
Abstract
The invention belongs to the technical field of tissue engineering, and discloses a method for preparing tissue engineering corneal limbus based on Matrigel. The method for preparing the tissue engineering corneal limbus based on the Matrigel comprises the steps that (a) freeze thawing is carried out on the Matrigel, and then icy balls or dry icy balls are added into the Matrigel for being stirredand mixed to obtain a solidliquid mixture; (b) the Matrigel in the solidliquid mixture is gelatinized, in the process, the icy balls or dry icy balls in the solidliquid mixture melt and leave pores in the gelatinized Matrigel, so that the obtain Matrigel has a porous structure; and (c) corneal limbus stem cells and corneal limbus micro-environment cells are cultivated in the Matrigel which is provided with the porous structure, so that the tissue engineering corneal limbus required by a three-dimensional structure is formed in the porous structure. The method for preparing the tissue engineering corneal limbus based on the Matrigel has the advantages of simple process, easy implementation, convenient control of technological conditions and the Matrigel porous structure and the like.
Year: 2019
Country: CN
Doc No: 109897823