23 Jul Method for preparing three-dimensional tubular multi-cellular structure
Abstract
The invention relates to a method for preparing a three-dimensional tubular multi-cellular structure and relates to the field of tissue engineering. According to the method, a composite hydrogel structure composed of upper hydrogel (1) and lower hydrogel (2) which have mechanical characteristics and different molecular diffusion rates is established, cells in the hydrogel are cultured for a long time, and then the cells are made to be organized automatically near the division surface between the upper hydrogel and the lower hydrogel so as to form the tubular structure (4) or the tubular structure (5). Under the initial condition, the cells wrapped by the upper hydrogel (1) and the lower hydrogel (2) are evenly distributed but have different densities. The three-dimensional tubular multi-cellular structure prepared through the method is independent of any external composite structures, and the communication capacity between the large number of cells is fully utilized; the forming process of the three-dimensional tubular multi-cellular structure is more similar to the natural forming process of biological tissue, rejection reactions of existing artificial tubular tissue produced through polymer can be greatly reduced, and the efficient, high-reliability and low-cost method for preparing artificial tubular tissue is provided.
Year: 2015
Country: CN
Doc No: 104906636