23 Jul Method for preparing three-dimensional silk fibroin porous scaffold material
Abstract
The invention discloses a method for preparing three-dimensional silk fibroin porous scaffold material, and relates to the field of biomaterial, in particular to the field of tissue engineering scaffolds. The method comprises: putting silk fibroin water solution with the volume concentration of 2-50wt% into a container, and adding sodium chloride into the silk fibroin water solution at 20-80 DEG C under continuous stirring, wherein the ratio between the mass (unit: gram) of sodium chloride granules and the volume (unit: ml) of silk fibroin solution is 1-20:1; after gelatinating, standing and precipitating the silk fibroin solution, taking out the precipitate to be continuously stirred and evenly mixed; then, putting the precipitate into a mould with the needed shape to be dried; finally, washing off the sodium chloride in the scaffold by distilled water, and obtaining the silk fibroin porous scaffold material having three-dimensional porous structure. No organic solvent is used in themethod, the method is simple and efficient, and the prepared silk fibroin porous scaffold has controllable porosity factor and pore size, highly communicated pore structure as well as degradability.
Year: 2009
Country: CN
Doc No: 101596327