23 Jul Method for preparing sulfated silk fibroin vascular tissue engineering scaffold by using electrostatic spinning
Abstract
The invention relates to a sulfated silk fibroin vascular tissue engineering scaffold prepared by using electrostatic spinning and belongs to the field of biological medical materials. The sulfated silk fibroin vascular tissue engineering scaffold is prepared through the following steps of: dissolving a silk fibroin and sulfated silk fibroin sponge in hexafluoroisopropanol, so as to prepare a 7-10% mixed silk fibroin and sulfated silk fibroin solution; filling the solution in an injector, applying a 10-30kV voltage, and receiving fibers by using a rotary metal rod, wherein the receiving distance is 10-25 cm, the diameter of the selected receiving rod is 3-5 mm, the rotating speed is 200-1000 rpm (revolutions per minute), and the propelling speed of the injector is 0.03-0.05 mm/s, so as to obtain a tubular tissue engineering scaffold with the thickness of 0.1-1 mm and the internal diameter of 3-5 mm; then inducing the silk fibroin to convert to a beta laminated structure by using a 90-100% methanol solution to soak the scaffold, so that the tissue engineering scaffold can be prevented from being dissolved in water by virtue of improving mechanical properties. The vascular tissue engineering scaffold prepared by the invention has excellent cell compatibility and long-term and stable anti-blood coagulation property, has controllable caliber, and can be used for maintenance of small-caliber blood vessels and establishment of tissue engineering and hemodialysis access. The preparation method provided by the invention has the advantages of simplicity in operation and low cost, and can be used for realizing commercial production.
Year: 2012
Country: CN
Doc No: 102652841