23 Jul Method for preparing skin tissue engineering scaffold based on 3D bio-printing technology and in-vitro cytotoxicity testing method for scaffold
Abstract
The invention discloses a method for preparing a skin tissue engineering scaffold based on a 3D bio-printing technology and an in-vitro cytotoxicity testing method for the scaffold. The method comprises the following process steps: preparing high-strength cellulose nanofiber/gelatin complex hydrogel for a printing scaffold, printing a 3D tissue engineering scaffold and cross-linking the scaffold.According to the method disclosed by the invention, the requirements on high porosity and high precision of the tissue engineering scaffold are met by utilizing the 3D bio-printing technology. The CNFserves as a filling material of the GEL and has an effect of improving the mechanical strength of the GEL, and the printed scaffold is soaked in a genipin solution to be cross-linked. The skin tissueengineering scaffold prepared by the method has excellent mechanical property, and does not have any toxic or side effect or immunologic rejection. Meanwhile, the tissue engineering scaffold preparedby using the 3D printing technology has the advantages of being convenient, rapid and easy to control, and can be subjected to personalized customization according to depths, sizes and shapes of wounds of patients.
Year: 2018
Country: CN
Doc No: 108114324