23 Jul Method for preparing prostaglandin E1 by using gene engineering cyclooxygenase-1 and gene engineering prostaglandin E synthetase-1
Abstract
The invention discloses a method for preparing prostaglandin E1 by using gene engineering cyclooxygenase-1 and gene engineering prostaglandin E synthetase-1, and belongs to the field of bioengineering. The method comprises the following steps: expressing related enzymes required by prostaglandin E1 synthesis by a prokaryotic expression means, namely cyclooxygenase-1 (COX-1) and prostaglandin E synthetase-1 (mPGES-1); and reacting the enzymes with a substrate to synthesize the prostaglandin E1. By the method, a large amount of prostaglandin synthetase can be expressed, the prostaglandin E1 is synthesized, and the problem that tissue enzymes taken from living bodies are easy to pollute in industrial production can be solved. Meanwhile, the enzyme type expressed by a prokaryotic expression issingle, the concentration of the expressed enzyme is high, organic impurities in an escherichia coli system are few, and the impurities of a product after enzymatic reaction are few, so that the purification and utilization of the prostaglandin E1 are facilitated, and a new path is opened up for the artificial synthesis of the prostaglandin E1.
Year: 2020
Country: CN
Doc No: 110951814