23 Jul Method for preparing polypeptide copolymer porous nanofiber by using electrostatic spinning
Abstract
The invention relates to a method for preparing polypeptide copolymer porous nanofiber by using electrostatic spinning. The method comprises the following steps: firstly, carrying out hydrophilic modification on a hydrophobic polypeptide homopolymer to prepare a poly(gamma-benzyl L-glutamate-hydroxyethyl glutamine) or poly(gamma-benzyl L-glutamine)-g-polyethylene glycol grafted copolymer; secondly, preparing a compound solvent by using solvents with different volatilization properties, and then preparing into a spinning solution; thirdly, spinning by using a high voltage electrostatic spinning method; and finally, placing spun yarns into a vacuum drying chamber and drying for more than 3 hours at room temperature to obtain the porous nanofiber with high specific surface area. A cytotoxicity test indicates that the prepared porous nanofiber with high specific surface area is free of cytotoxicity and good in biocompatibility, can be used as a porous scaffold for cell growth, has the function of promoting cell migration and amplification and has a relatively good application value in the aspect of preparation of scaffolds for tissue engineering; besides, the polypeptide copolymer porous nanofiber has an application prospect in the fields of medical dressing, medicine released control, artificial organs, sewage treatment and the like.
Year: 2014
Country: CN
Doc No: 103590133