23 Jul Method for preparing multilayer spiral urethral tissue engineering scaffold
Abstract
The invention discloses a method for preparing a multilayer spiral urethral tissue engineering scaffold. A thermoplastic degradable polymer is used as a printing raw material, a rectangular strip embryoid body with hollow patterns by adopting a fused deposition type 3D printer, an embryoid body spiral tube is obtained by spirally curling, and after heat treatment and nesting, a double-layer scaffold consisting of an inner-layer embryoid body spiral tube and an outer-layer embryoid body spiral tube is obtained, one end of the double-layer scaffold is sewed by a polylactic acid suture, an endothelial cell sheet layer and a urothelial cell sheet layer which are transfected with vascular endothelial growth factors and fibroblast growth factors during culture and a smooth muscle cell sheet layer which is transfected with human platelet growth factors during culture are lined on the inner wall of the outer embryoid body spiral tube in sequence, extracellular matrix is filled, the other end of the double-layer scaffold is sewed, and the urethral tissue engineering scaffold can be obtained after thrombin is fixed. The scaffold prepared by the invention can keep a urethra smooth, is beneficial to healing the injured urethra tissue, realizes the vascularization of a urethra scaffold in time and can quickly realize the revascularization.
Year: 2020
Country: CN
Doc No: 110680961