23 Jul Method for preparing injectable chitosan/sodium glycerophosphate/collagen hydrogel
Abstract
The invention discloses a method for preparing injectable chitosan/sodium glycero-phosphate/collagen hydrogel, belonging to the field of biotechnology and tissue engineering; the invention particularly relates to a technology for preparing temperature sensitive type injectable material. The method is characterized in that 2.2% (W/V) of chitosan solution is mixed with 50% (W/V) of beta-sodium glycero-phosphate solution at low temperature to prepare chitosan/sodium glycero-phosphate solution; then, the solution is mixed with collagen solution following a volume ratio of 1:10-10:1 at low temperature; the mixture is retained in the liquid state at room temperature( 8 DEG C) and is turned into gel at body temperature within 12min. The invention has the beneficial effects that the chitosan/sodium glycero-phosphate/ collagen hydrogel can be maintained in the liquid state at low temperature when pH value is within physiological range (pH value ranges from 7.0 to 7.2); the mixture is turned into gel when the temperature is raised to body temperature (37 DEG C). As ideal cell growth substrate collagen is added, the hydrogel features fine biocompatibility.
Year: 2009
Country: CN
Doc No: 101502673