23 Jul Method for preparing heparin collagen/chitosan porous rack of composite angiogenin
Abstract
The preparation process of porous heparinized collagen/chitosan rack with compounded angiogenin includes the following steps: dissolving ox tendon and chitosan separately in acetic acid solution to compound 0.5-5 % concentration solution, mixing chitosan solution in 5-30 % and ox tendon solution, molding and freeze drying to obtain porous collagen/chitosan rack; vacuum treatment, soaking the porous collagen/chitosan rack inside 2-N-morpholynyl ethane sulfonic acid solution of heparin sodium, treating with 1-ethyl-3-3-(dimethylaminopropyl)-carbonized diimine solution and N-hydroxy batanimide solution, washing, re-freezing to dry and to obtain porous heparinized collagen/chitosan rack; and soaking the porous heparinized collagen/chitosan rack inside angiogenin solution to obtain the porous heparinized collagen/chitosan rack with compounded angiogenin. The prepared rack has proper pore size and porosity and is used as the corium substitute in skin tissue engineering.
Year: 2006
Country: CN
Doc No: 1748803