23 Jul Method for preparing epithelium of autologous cornea
Abstract
This invention discloses a method for preparing auto-corneal epithelia. The method comprises: (1) performing primary culture of cells of patients’ oral mucosa tissue or corneal limbal tissue for 3-15 days; (2) performing proliferation sub-culture for 7-30 days; (3) using fibrinogen as the supporting material to construct tissue engineering corneal epithelia together with the proliferation sub-cultured cells. The method can avoid the problems of immunological rejection, pollution, ununiform cell growth caused by the biological support, and slow degradation faced by presents techniques. The preparation of auto-corneal in this invention is not restricted by corneal epithelia damage, and suitable corneal is available for patients at any situation.
Year: 2007
Country: CN
Doc No: 1916166