23 Jul Method for preparing decellularized lamellar cornea matrix sheet
Abstract
The invention discloses a method for preparing a decellularized lamellar cornea matrix sheet. The method comprises the steps of disinfecting a fresh animal eyeball; treating with filter paper dipped with alcohol after disinfecting, and then erasing an epithelial cell layer; incising the corneal limbus under an operation microscope, stretching into an iris restorer to separate the anterior cornea, and then shearing the anterior cornea with corneal scissors; drilling the lamellar cornea with a corneal annulus; soaking the fresh lamellar cornea into serum, and then rinsing or performing ice-bath electrophoresis treatment; performing gradient dehydration after the treatment to obtain a non-dried lamellar tissue engineering corneal frame, sterilizing with ethylene oxide, and preserving for later use; drying the non-dried cornea matrix sheet in a 24-pore plate to obtain dried decellularized lamellar cornea; and sterilizing the dried sample with cobalt 60, and performing rehydration treatment to obtain a rehydrated lamellar cornea matrix sheet. The obtained cornea matrix sheet is high in transparency, low in structural destroy, good in biocompatibility, close to fresh cornea in performance and thorough in decellularization.
Year: 2015
Country: CN
Doc No: 104645415