23 Jul Method for preparing cross-linked collagen/chitosan tissue engineering porous support in one-step freeze-drying mode
Abstract
The invention discloses a method for preparing a cross-linked collagen/chitosan tissue engineering porous support in one-step freeze-drying mode, which uses natural biological material of collagen and chitosan as raw materials, directly adds collagen/chitosan mixed swelling solutions in a glutaraldehyde solution and prepares the collagen/chitosan tissue engineering porous support through a one-step refrigeration freeze-drying mode. The collagen/chitosan support prepared through the method has the advantages of being controllable in mechanical property, proper in degradation rate and good in biocompatiability. Further, the method only needs a one-step freeze-drying process and effectively solves the problems of support collapsing, micro-structure breaking and the like caused by a traditional preparing method. The method is simple and practical, low in energy consumption, time-saving, material-saving and good in repeatability, and the constructed collagen/chitosan porous support can be widely used in the field of tissue engineering and has good clinical application prospect.
Year: 2012
Country: CN
Doc No: 102580163