23 Jul Method for preparing corneal epithelium form epidermis stem cell through fabrication of tissue engineering, and application
Abstract
This invention discloses the preparation for artificial corneal epithelium implants made up of skin dry cells and a usage in transplanting and repairing a blind eye skinsbecause of defecting limbus corneae dry cells. The artificial corneal epithelium implant applies skins of adult neck or milk goat ear edge in asepsis to purify skin dry cells with TC and clone selection, amplification with serum-free medium and detect the got dry cells with CK19, P63, integrin-beta1, label protein immune tissue then to vaccinate the purified dry cells on to human amnion frame of depithelial cell nurtured by serum-free medium for 21days. The cells are separated on the amnion to form stratified layers and become cell amnion implants. The cells are divided into 5-6 layers having the structure of normal cornea epithelia.
Year: 2005
Country: CN
Doc No: 1563366