23 Jul Method for preparing conductive composite nanofiber nervous tissue engineering scaffold based on graphene
Abstract
The invention provides a method for preparing a conductive composite nanofiber nervous tissue engineering scaffold based on graphene. The method includes the following steps: a step 1, dissolving tussur silk fibroin and poly (lactic acid)-poly caprolactone in a solvent with stirring till complete dissolution of the tussur silk fibroin and the poly (lactic acid)-poly caprolactone so as to acquiring a spinning solution; a step 2, performing electrostatic spinning on the spinning solution obtained from the step 1 so as to acquire a nanofiber membrane, performing steam fumigation treatment by using ethyl alcohol, and performing drying so as to acquire tussur silk fibroin/ poly (lactic acid)-poly caprolactone composite nanofibers; and a step 3, dipping the tussur silk fibroin/ poly (lactic acid)-poly caprolactone composite nanofiber scaffold material obtained from the step 2 in a graphene oxide dispersion liquid, taking out the tussur silk fibroin/ poly (lactic acid)-poly caprolactone composite nanofiber scaffold material, cleaning the tussur silk fibroin/ poly (lactic acid)-poly caprolactone composite nanofiber scaffold material, soaking the tussur silk fibroin/ poly (lactic acid)-poly caprolactone composite nanofiber scaffold material in an ascorbic acid solution, taking out the tussur silk fibroin/ poly (lactic acid)-poly caprolactone composite nanofiber scaffold material, and cleaning the tussur silk fibroin/ poly (lactic acid)-poly caprolactone composite nanofiber scaffold material so as to acquiring the conductive composite nanofiber nervous tissue engineering scaffold based on the graphene. The method is simple to operate, is excellent in repeatability, and can provide a new thought for nerve defect repairing.
Year: 2017
Country: CN
Doc No: 106400312