23 Jul Method for preparing complicated tissue organ precursor with multilayer structure
Abstract
The invention relates to a method for preparing a complicated tissue organ precursor with a multilayer structure. The method comprises the following steps: preparing synthetic macromolecular solution and cell matrix solution and injecting the synthetic macromolecular solution into an inner die to form an inner bracket; sleeving at least two middle dies at the outer part of the inner bracket and injecting the cell matrix solution between the inner bracket and the middle dies to form a cell matrix middle layer; forming a double-layer structure of the inner bracket and a cell matrix by physical or chemical crosslinking; placing the double-layer structure into an outer die and injecting the cell matrix solution into a gap between the double-layer structure and the outer die to form an outer-layer synthetic macromolecular material layer; extracting solvent in the synthetic macromolecular material layer by cell culture solution and removing the dies. The invention can accurately position different cell matrix materials and a synthetic macromolecular bracket in a space and overcome the defects of long time of culture cell induction, uneven cell distribution, difficult cell infiltration in a deep-layer structure, and the like in a three-dimensional bracket in tissue engineering.
Year: 2010
Country: CN
Doc No: 101623515