23 Jul Method for preparing cell culture vector
Abstract
The invention relates to a method for preparing a cell culture vector, which comprises the following steps: firstly, preparing nanoscale silk fibroin powder; secondly, dispersing or dissolving the nanoscale silk fibroin powder in an aqueous solution to blend with an aqueous solution of polyvinyl alcohol, and forming the blending solution into a fiber or a membrane; and finally, making the fiber or the membrane into a required three-dimensional shape to obtain the cell culture vector. The preparation method has the advantage of easy industrial application; and cells cultured by the obtained cell culture vector have the characteristics of high density, high yield and low cost. The cell culture vector prepared by the method is used for culturing target cells, and the cultured target cells can be used for gene expression and preparation of genomic medicaments. In addition, the cell culture vector also can be used for a tissue engineering bracket.
Year: 2009
Country: CN
Doc No: 101603007