23 Jul Method for preparing CaSiO<3>@SiO<2> particles containing immobilized enzymes
Abstract
The invention provides a method for preparing CaSiO<3>@SiO<2> particles containing immobilized enzymes. The method for preparing the CaSiO<3>@SiO<2> particles containing the immobilized enzymes includes enabling sodium silicate to be reacted with calcium ions to prepare calcium silicate; treating the calcium silicate by hydrochloric acid to obtain CaSiO<3>@SiO<2> particles containing grafting mesoporous silica gel on surfaces; and immobilizing enzymes on the calcium silicate particles containing the mesoporous-structured silica gel on surfaces, namely the CaSiO<3>@SiO<2> particles, by physical absorption and covalent bond immobilizing processes. The CaSiO<3>@SiO<2> particles containing the immobilized enzymes can be grafted to surfaces of matrix materials with different shapes to obtain materials with the CaSiO<3>@SiO<2> containing the immobilized enzymes on surfaces. The method is simple in operation, organic solvents are omitted, the immobilized enzymes are high in activity, the materials are good in biocompatibility and can be degraded in bodies of users, and the method has an excellent application prospect in fields of tissue engineering, catalysis and the like.
Year: 2013
Country: CN
Doc No: 103013975