23 Jul Method for preparing blood vessel inner layer scaffold material used for biodegradable tissue engineering
Abstract
The invention discloses a method for preparing blood vessel inner layer scaffold material used for biodegradable tissue engineering, comprising the steps: human-like collagen is dissolved to be 1-3% of solution by distilled water; hyaluronic acid is dissolved to be 0.5-2% of solution by distilled water; the two obtained solutions are evenly mixed together, mixed with glutaric dialdehyde water solution with the mass percent concentration of 20-50% and stirred; after being filtered, the obtained solution is processed by vacuum defoamation; the mixed solution is injected into a tubular shape mould to be processed by cross linkage at the temperature of 4 DEG C for 1-3 days, and then put into a refrigerator with the temperature of -80 DEG C to be frozen and dried, so that the blood vessel inner layer scaffold is prepared. Compared with the prior art, the prepared scaffold material has excellent mechanical capacity, biocompatibility, blood compatibility and immunity compatibility as well as lower immunity rejection reaction, thoroughly avoids the virus hidden trouble which is inevitable for animal collagen, and greatly improves the safety.
Year: 2009
Country: CN
Doc No: 101554489