23 Jul Method for preparing acellular ligament or tendon stent
Abstract
The invention relates to the technical field of tissue engineering, in particular to a method for preparing an acellular ligament or tendon stent. In the conventional method for preparing the ligament or tendon acellular ligament stent, acellular treatment is performed by jointly applying physical, chemical and enzyme methods, wherein the use of a detergent is an important step in the process of the acellular treatment. Ligaments and tendons are compact connective tissues and the inside and the outside of the fiber bundle are wrapped by an inner membrane and an outer membrane to make cellularconstituents difficult to remove, so that the acellular period is long and the acellular process causes large damage to an extracellular matrix. The invention provides the method for preparing the acellular ligament or tendon stent, and in the method, laser treatment is performed on the surfaces of ligaments or tendons by using a dot matrix with suitable density and energy and then few detergent or no detergent can be used according to the conditions in the process of acellular treatment, so that influence on the extracellular matrix is reduced and adhesion and growth of the cells are facilitated.
Year: 2010
Country: CN
Doc No: 101829360