23 Jul Method for photochemical cross-linking cell adhesion peptides on chitosan hydroxyl
Abstract
The invention relates to a method for photochemical cross-linking cell adhesion peptides on chitosan hydroxyl, namely a group selectively grafts the cell adhesion peptides on chitosan molecules. In particular, bifunctional photosensitive crosslinker sulfo-SANPAH is utilized to modify hydrophilic polypeptide GRGDY containing cell adhesion factors RGD on the chitosan hydroxyl to increase the water solubility and the targeting of a material, while amidogen is retained and tedious amino protection, a deprotection process and chemical toxicity caused by the same are avoided. The method adopts UV-activated photochemical cross-linking and has the advantages of mild reaction conditions and fast reaction rate, and the feature group of the crosslinker is beneficial to judging the conducting of grafting reaction. The targeted adhesion chitosan material synthesized by the method is suitable for preparing DNA and small RNA (siRNA, microRNA, shRNA, and the like.) targeted delivery systems, cell adhesion cultures, microencapsulated tissue cells for transplant, scaffolds for tissue engineering, and the like applied in the biomedical field under physiological conditions.
Year: 2010
Country: CN
Doc No: 101747450